ABSTRACT

This book is a guide for users of new technologies, as it includes accurately proven protocols, allowing readers to prepare their samples for experiments. Additionally, it is a guide for the bioinformatics tools that are available for the analysis of the obtained tags, including the design of the software, the sources and the Web. Finally, the book provides examples of the application of these technologies to identify promoters, annotate genomes, identify new RNAs and reconstruct models of transcriptional control. Although examples mainly concern mammalians, the discussion expands to other groups of eukaryotes, where these approaches are complementing genome sequencing.

chapter Chapter One|6 pages

Cap Analysis Gene Expression (CAGE)

chapter Chapter Two|14 pages

Tagging Transcription Starting Sites with CAGE

chapter Chapter Three|20 pages

Construction of CAGE Libraries

chapter Chapter Five|18 pages

New Era of Genome-Wide Gene Expression Analysis

chapter Chapter Seven|10 pages

Extraction and Quality Control of CAGE Tags

chapter Chapter Ten|14 pages

Databases for CAGE Visualization and Analysis

chapter Chapter Fourteen|18 pages

Lessons Learned from Genomic CAGE

chapter Chapter Fifteen|12 pages

Future Challenges in CAGE Analysis