ABSTRACT

In Situ hybridization allows the visualization of specific DNA/RNA sequences in individual cells in tissue sections, single cells, or chromosome preparations, and is an especially important method for studying DNA and RNA in heterogeneous cell populations. This book delves into in situ hybridization methods through the use of light microscopy used

chapter 1|60 pages

Probes

chapter 2|28 pages

Tissue Preparation

chapter 3|18 pages

Pretreatments

chapter 4|32 pages

Hybridization

chapter 5|48 pages

Revelation of Hybrids

chapter 6|20 pages

Coutnersaining and Modes of Observation

chapter 7|14 pages

Controls and Problems

chapter 8|40 pages

Typical Protocols