ABSTRACT

A technique used to amplify the number of copies of a specific region of DNA, the polymerase chain reaction (PCR) is at the forefront of the dramatic development of biochemistry. This text provides the tools for developing innovative approaches to using this leading technology. It includes theoretical considerations, discussions, and a selection of

chapter |4 pages

Section I: General Considerations

chapter 2|12 pages

Long PCR from Damaged DNA

chapter 5|14 pages

Pre-PCR Processing Strategies

chapter |4 pages

Section II: Analytical Applications

chapter 10|14 pages

High-Throughput Genotyping and the PCR

chapter 18|10 pages

Pyrosequencing Technology

chapter 19|8 pages

Octamer-Primed Cycle Sequencing

chapter 20|10 pages

Amplification of cDNA

chapter 29|10 pages

Suppression Subtractive Hybridization PCR

chapter |4 pages

Section III: Preparative Application

chapter 31|6 pages

Chromosome Walking by Inverse PCR

chapter 32|8 pages

Long Universal Fast Walking