ABSTRACT

Since the word microscopy was coined in 1656, the evolution of the instrument has had a long and convoluted history. Plagued with problems of chromatic aberration, spherical aberration, and challenges with illumination and resolution, the microscope's technical progression happened in a series of fits and starts until the late 19th century. After E

chapter 1|18 pages

Appendix

chapter 2|10 pages

Optical Contrasting Techniques

chapter 3|14 pages

Fluorescence and Fluorescence Microscopy

chapter 4|14 pages

Image Capture

chapter 5|20 pages

The Scanning Optical Microscope

chapter 6|10 pages

Contrast

chapter 7|14 pages

Geometrical Aberrations

chapter 8|14 pages

Multiphoton Microscopy

chapter 10|14 pages

Deconvolution and Image Processing

chapter 11|18 pages

Surfaces: Two-and-a-Half Dimensions

chapter 12|10 pages

Structure and Properties of GFP

chapter 13|12 pages

Immunolabeling

chapter 14|14 pages

Fluorescence Intensity Measurements

chapter 15|18 pages

Fluorescence Lifetime

chapter 16|14 pages

Near-Field Microscopy