ABSTRACT

Although the polymerase chain reaction has revolutionized genetic analysis by amplifying rare nucleic acid sequences, the in situ application is the only method that allows the localization of amplified signal within tissue structure. The applications of in situ polymerase chain reaction have greatly enhanced the field of investigation in many disc

chapter 1|2 pages

Chapter 1

General Principles

chapter |2 pages

CONTENTS

chapter 1|6 pages

1 POLYMERASE CHAIN REACTION (PCR)

chapter 1|2 pages

3 IN SITU PCR/RT-PCR

chapter 1|8 pages

4 DIRECT IN SITU PCR/RT-PCR

chapter 2|2 pages

Chapter 2

Preparation of Samples

chapter |24 pages

CONTENTS

chapter 3|2 pages

Chapter 3

Pretreatments

chapter |4 pages

CONTENTS

chapter 3|6 pages

3 DEWAXING

3.3.1

chapter 3|3 pages

6 POSTFIXATION

3.6.1

chapter 3|1 pages

8 DEHYDRATION

3.8.1

chapter 4|2 pages

- Reverse Transcription (RT)

Reverse Transcription

chapter |3 pages

CONTENTS

chapter 4|7 pages

2 DIAGRAM OF THE DIFFERENT STEPS 4.3 THE TOOLS

4.3.1 Primers

chapter 4|10 pages

4 MATERIALS/REAGENTS/SOLUTIONS

4.4.1 Thermocycler

chapter 5|2 pages

- Polymerase Chain Reation (PCR)

Polymerase Chain Reaction (PCR)

chapter |6 pages

CONTENTS

chapter 5|1 pages

2 DIAGRAM OF THE DIFFERENT STEPS

chapter 5|23 pages

3 TOOLS

5.3.1 Deoxynucleotide Triphosphates (dNTP)

chapter 6|2 pages

Chapter 6

Hybridization

chapter |4 pages

CONTENTS

chapter 6|13 pages

3 TOOLS: THE PROBES

chapter 6|7 pages

4 HYBRIDIZATION PARAMETERS

chapter 6|2 pages

7 BEFORE REVELATION

6.7.1 Radioacti Hybrids

chapter 7|2 pages

Chapter 7

Revelation

chapter |28 pages

CONTENTS

chapter 8|2 pages

Chapter 8

Electron Microscopy

chapter |4 pages

CONTENTS

chapter 8|12 pages

1 OVERVIEW

8.1.1 Objective

chapter 8|16 pages

3 SAMPLES

8.3.1 Origin

chapter 8|22 pages

8 PCR

8.8.1 Overview

chapter 9|2 pages

Chapter 9

Controls and Problems

chapter |21 pages

CONTENTS

chapter 9|21 pages

3 CONTROLS

9.3.1 Tools and Reagents

chapter 10|2 pages

Chapter 10

Typical Protocols

chapter |10 pages

CONTENTS

chapter 10|34 pages

2 INDIRECT IN SITU PCR

chapter 11|2 pages

Chapter 11

Examples of Observations

chapter |1 pages

FIGURE11 . 1 2

chapter |1 pages

FIGURE11 . 1 3

chapter |1 pages

FIGURE11 . 1 4

chapter |1 pages

FIGURE11 . 1 5

chapter |1 pages

FIGURE11 . 1 6

chapter |2 pages

FIGURE11 . 1 7

part |2 pages

Appendices

chapter |4 pages

Appendix A

EQUIPMENT PRACTICAL PRECAUTIONS

chapter |16 pages

Appendix B

REAGENTS WATER

chapter |15 pages

B4 FIXATIVES

chapter |3 pages

B8 MOUNTING MEDIA

Aqueous